尚画雨,白胜超,夏志,周越,王瑞元,等.运动诱导骨骼肌损伤对大鼠骨骼肌线粒体结构和功能的影响[J].北京体育大学学报,2018,41(1):58-63.
运动诱导骨骼肌损伤对大鼠骨骼肌线粒体结构和功能的影响
Effect of Exercise-Induced Skeletal Muscle Damage on Mitochondrial Structure and Function in Skeletal Muscle of Rats
投稿时间:2017-03-16  
DOI:10.19582/j.cnki.11-3785/g8.2018.01.008
中文关键词:  关键词:大负荷运动  骨骼肌  线粒体  超微结构  呼吸链复合体  CS
英文关键词:Keywords: heavy load exercise  skeletal muscle  mitochondria  ultrastructure  respiratory chain complex  CS
基金项目:基金项目:国家自然基金资助项目(编号:31471133);成都体育学院校级科研项目(编号:17YJ04);北京体育大学重点实验室项目基金项目:国家自然基金资助项目(编号:31471133);成都体育学院校级科研项目(编号:17YJ04);北京体育大学重点实验室项目(2014SYS005)。通信作者:王瑞元。(2014SYS005)。
作者单位
尚画雨 成都体育学院运动医学与健康学院四川 成都 610041北京体育大学运动人体科学学院北京 100084 
白胜超 北京体育大学运动人体科学学院北京 100084 
夏志 井冈山大学体育学院江西 吉安 343009 
周越 北京体育大学运动人体科学学院北京 100084 
王瑞元 北京体育大学运动人体科学学院北京 100084 
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中文摘要:
      摘要:目的:探讨大负荷运动对大鼠骨骼肌线粒体形态结构与功能的影响,为研究运动性骨骼肌损伤发生的机理奠定基础。方法:48只成年雄性Sprague-Dawley大鼠,随机分为安静对照组(C,n=8)和运动组(E,n=40)。其中,E组按时相点划分为0 h、12 h、24 h、48 h、72 h等5组,每组各8只。E组大鼠在跑台上进行持续性下坡跑,跑台坡度为-16°,速度为16 m/min,运动时间为90 min。各组分别于对应时间点分离比目鱼肌进行检测。使用透射电子显微镜观察骨骼肌线粒体超微结构变化,采用ELISA方法检测各组大鼠比目鱼肌线粒体关键酶CS的含量以及线粒体呼吸链复合体Ⅱ、Ⅳ的活性,应用Western blot方法检测骨骼肌COXⅠ的蛋白表达。结果:一次大负荷运动后比目鱼肌线粒体出现明显肿胀、肌膜下积聚等超微结构异常变化,且伴有大量不同成熟阶段的自噬体形成,同时CS的含量和呼吸链复合体Ⅱ活性均明显下调(P<0.05),复合体Ⅳ活性及其亚基COXⅠ蛋白表达也出现下降。结论:一次大负荷运动可导致骨骼肌线粒体结构和功能受损,数量减少,导致细胞氧化磷酸化功能障碍,同时还诱导了自噬的发生,这可能是大负荷运动致骨骼肌损伤的重要原因之一。
英文摘要:
      Abstract: Objective: The purpose of this study was to explore the effect of heavy load exercise on mitochondrial structure and function in skeletal muscle of rats,?and to lay the foundation for studying the mechanism of exercise-induced skeletal muscle injury. Methods: Forty-eight male adult SD rats were randomly divided into quiet control group (C group, n=8) and exercise group (E group, n=40). E group was further divided into 0h, 12h, 24h, 48h and 72h sub-groups (n=8). Rats in the E group performed 90 min downhill running on treadmill at speed of 16 m/min, the slope of running platform was -16o. The soleus muscle was collected under anesthesia at the designed time. The changes of ultrastructure of skeletal muscle mitochondria was observed by a transmission electron microscope, the content of citrate synthase(CS) and the activities of mitochondrial respiratory chain complex Ⅱand Ⅳwere measured by ELISA, and the protein expression of skeletal muscle COXⅠwas detected by Western blot. Results: After heavy load exercise, the mitochondrial structure appeared changes such as swelling, accumulating under cell membrane, and forming a large number of autophagosomes; the content of CS and the activity of mitochondrial respiratory chain complex Ⅱ significantly decreased (P < 0.05); and the activity of complex Ⅳ and the expression of COXⅠalso decreased. Conclusion: A heavy load exercise can lead to the structure and function damage of mitochondria in skeletal muscle, reduce the number of mitochondria, lead to oxidative phosphorylation dysfunction, and induce autophagy, which may be one of the important reasons of heavy load exericise-induced skeletal muscle damage.
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