杨永钟,许思毛,王春亮.SarcKATP介导运动预处理对I/R心脏的保护作用[J].北京体育大学学报,2017,40(3):52-59.
SarcKATP介导运动预处理对I/R心脏的保护作用
Protective Effect of Exercise Preconditioning Mediated by SarcKATP on I/R Heart
投稿时间:2016-03-21  
DOI:
中文关键词:  关键词:运动预处理  心肌缺血再灌注损伤  细胞膜ATP敏感性钾通道  心电图  心功能
英文关键词:Keywords:exercise preconditioning  myocardial ischemia reperfusion injury  sarcolemmal ATP-sensitive potassium channel  ECG  cardiac function
基金项目:基金项目:广西自然科学基金项目(2013GXNSFBA019146)。通信作者:王春亮
作者单位
杨永钟 长江师范学院体育与健康科学学院重庆 408100 
许思毛 广西师范大学体育学院广西 桂林 541004 
王春亮 广西师范大学体育学院广西 桂林 541004 
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中文摘要:
      摘要:目的:探讨细胞膜ATP 敏感性钾通道(sarcKATP)在较大强度运动预处理保护缺血再灌注(I/R)心脏中的作用及其与 Kir6.2 亚基蛋白表达变化的关系。方法:108只成年雄性 SD 大鼠,随机分为安慰剂后假手术对照组(PS)、抑制剂后假手术对照组(DS),运动与安慰剂后假手术对照组(EPS)、运动与抑制剂后假手术对照组(EDS),安慰剂后I/R组(PI)、抑制剂后I/R组(DI),运动与安慰剂后I/R组(EPI)、运动与抑制剂后I/R组(EDI)。除PS、ES、EPS、EDS组进行假手术外,其余4组均建立在体I/R(缺血30 min、再灌注60 min)心脏模型;且术前分别采用对应方式进行干预。观察心电图及左室内压变化,每组随机取8只实验成功者进入后续实验,检测血液cTnI、CK-MB浓度及心肌sarcKATP中Kir6.2亚基蛋白表达水平。结果:与缺血前相比,缺血30 min时各I/R组心电图J点及T波幅度显著增加、Q-T间期显著延长(P<0.05),再灌注60 min时的J点及T波值显著回落(P<0.05)。与 PS 组相比,各运动组术前LVSP与±dp/dtmax 显著升高、LVEDP 显著下降(P<0.05)。与缺血前相比,缺血30 min时各 I/R组 LVSP与 ±dp/dtmax 显著下降、LVEDP 显著升高(P<0.05),而再灌注60 min 时的异常变化较缺血30 min 时更明显(P<0.05);与PS组相比,各I/R组术后血液 cTnI、CK-MB浓度显著升高(P<0.05)。对I/R引起的上述心电图及左室内压参数、血液指标等异常程度进行组间比较,DI组大于PI组、PI组大于EPI组、EDI组大于 EPI 组(P<0.05)。此外,运动引起心肌sarcKATP中Kir6.2蛋白表达水平增加。结论:运动锻炼提高了心肌功能,而SarcKATP介导了运动预处理对I/R心脏的保护作用,并可能是通过诱导其亚基Kir6.2的蛋白表达即增加孔道量来实现。
英文摘要:
      Abstract: Objective: The purposes of this study were to explore the role of Sarcolemmal ATP-Sensitive Potassium Channels (sarcKATP) in protective effect of vigorous intensity exercise precondition against ischemia reperfusion damage of heart, and to detect its correlation with Kir6.2. Methods: One hundred and eight male adult SD rats were randomly divided into sham-operation after placebo control group (PS), sham-operation after inhibitor control group (DS), sham-operation after exercise and placebo control group (EPS), sham-operation after exercise and inhibitor control group (EDS), ischemia reperfusion (I/R) after placebo group (PI), I/R after inhibitor group(DI), I/R after exercise and placebo group (EPI), I/R after exercise and inhibitor group (EDI). Group PS, ES, EPS, EDS were sham operated, group PI, DI, EPI, EDI were build I/R (30 min ischemia, 60 min reperfusion) heart model, and each group was carried on the corresponding intervention. Rats’ ECG and left intraventricular pressure (LVSP, LVEDP) were observed; 8 rat of each group were selected and finished the following experiment, their serum cardiac troponin-IcTnI, creatine kinase rsoenzyme (CK-MB) and Kir6.2 subunit of sarcKATP were tested. Results: J point, T wave value and Q-T segment of ECG significantly increased after 30 min myocardial ischemia in PI, DI, EPI, EDI groups (P < 0.05), and J point and T wave were significantly recovered after 60 min reperfusion (P < 0.05). Compared with group PS, LVSP ± dp/dtmax significantly increased before operation in exercise groups, and LVEDP significantly decreased (P < 0.05). After 30 min ischemia, -LVSP and ±dp/dtmax of I/R groups significantly decreased while LVEDP increased (P < 0.05), and they changed more at 60 min reperfusion (P < 0.05). Compared with group PS,concentrations of blood cTnI and CK-MB significantly increased in I/R groups after operation (P < 0.05). The abnormal degrees of ECG and left ventricular pressure parameters, blood indicators caused by I/R of group DI were larger than those of group PI, and those of group PI and group EPI were larger than group EPI.In addition, exercise increase Kir6.2 protein expression in myocardial sarcKATP.Conclusion: Exercise improves cardiac function; sarcKATP plays a role in the exercise preconditioning against I/R damage of heart, and it may be realized by increasing Kir6.2 protein level (channel number)in sarcKATP.
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