陈婷,谭支内,冯瑞,田振军,等.早期氢水和运动干预对心梗心脏β3-AR表征研究[J].北京体育大学学报,2016,39(9):63-69.
早期氢水和运动干预对心梗心脏β3-AR表征研究
Effect of Hydrogen-Rich Water and Exercise Prior to Myocardial Infarction on β3-AR of Left Ventricular in Rats
投稿时间:2015-02-05  
DOI:10.19582/j.cnki.11-3785/g8.2016.09.010
中文关键词:  关键词:氢水干预  早期运动  心脏β3-AR  心肌梗死  内皮型一氧化氮合酶
英文关键词:Keywords: hydrogen water  early exercise  β3-AR of heart  myocardial infarction  eNOS
基金项目:基金项目:国家自然科学基金资助项目(编号:81360297, 31371199)。通信作者:田振军。
作者单位
陈婷 西藏民族学院陕西 咸阳 712082陕西师范大学陕西 西安 710062 
谭支内 陕西师范大学陕西 西安 710062 
冯瑞 陕西师范大学陕西 西安 710062 
田振军 陕西师范大学陕西 西安 710062 
摘要点击次数: 2211
全文下载次数: 2
中文摘要:
      摘要:目的:探讨早期氢水和有氧运动干预对心肌梗死大鼠左心室β3-AR及其下游分子eNOS蛋白表达的影响。方法: 雄性SD大鼠随机分为假手术组(S)、心肌梗死组(MI)、有氧运动+心梗组(EM)、氢水+心梗组(HM)和氢水+有氧运动+心梗组(HEM)。HM和HEM组大鼠均给予早期氢水灌胃4 mL,灌胃时间5 d/周×3周。EM和HEM组大鼠均进行早期3周递增式跑台运动。第1周适应性训练,时间20 min/d,速度15 m/min;第2周运动时间为25~30 min/d,速度为27~30 m/min;第3周运动时间为60 min/d,速度为30 m/min,5 d/周×3周。3周氢水及运动干预后MI,EM,HM和HEM组大鼠均结扎心脏左冠状动脉前降支,建立MI模型。S组大鼠实施假手术。5组大鼠术后45 min测定血流动力学指标判定心功能变化。心脏Masson染色测定胶原容积百分比(CVF)。采用荧光免疫组化法检测左心室β3-AR蛋白表达。采用western blot法检测β3-AR及下游eNOS蛋白水平。结果: MI后心脏CVF值显著升高(P<0.01),心功能显著降低,同时左心室β3-AR蛋白表达有增加趋势。与MI组比较,EM、HM和HEM组CVF(P<0.01)均显著降低,心功能均得到提升。EM、HM和HEM组均可见左心室β3-AR阳性染色,同时EM和HM组β3-AR和eNOS蛋白表达均较MI组显著增加(均P<0.05),HEM组β3-AR和eNOS表达较MI组有增加趋势。且左心室β3-AR与eNOS蛋白表达呈显著正相关(R=0.47, P<0.01)。结论: 心梗之前进行3周氢水和运动干预可减轻心肌纤维化程度,提升心梗大鼠心功能,增加左心室β3-AR表达,上调其下游eNOS蛋白表达。氢水联合运动干预对β3-AR影响并不及单一干预效果。而3种干预方式对心功能提升并无差异。
英文摘要:
      Abstract: Objectives: To discuss the effect of hydrogen-rich water and exercise prior to myocardial infarction on β3-AR and eNOS of left ventricular in rats. Methods: Sixty male Sprague-Dawley rats were assigned to five groups: sham group (S), myocardial infarction group (MI), aerobic exercise + myocardial infarction group (EM), hydrogen-rich water + myocardial infarction group (HM), and aerobic exercise + hydrogen-rich water + myocardial infarction group (HEM). HM and HEM groups were suffered intragastric administration of 4 ml hydrogen-water, 5d/week, 3 weeks in total. EM and HEM groups finished 3 weeks incremental treadmill exercise: the 1st week: 15 m/min, 20 min/d, 5d/week; the 2nd week: 27-30m/min, 25-30min/d, 5d/week; the 3rd week: 30m/min, 60min/d, 5d/week. After 3 weeks, MI was induced by ligation of the left anterior descending coronary artery in all groups except S, S group was operated with sham operation. The hemodynamic parameters were measured to detect cardiac function 45 min after operation. The CVF was tested by Masson staining. Cardiac β3-AR was detected by IFC and WB, and cardiac eNOS expression was detected by WB. Results: After MI, CVF increased (P < 0.01), cardiac function decreased, cardiac β3-AR protein expression had an increasing trend. Compared with MI group, CVF of EM, HM and HEM groups significantly reduced (P < 0.01), cardiac function was improved, and cardiac β3-AR was positively stained. Compared with MI group, β3-AR and eNOS expressions of EM and HM were higher (P < 0.05), and it had higher trend of HEM. β3-AR expression was positively correlated with eNOS protein expression (r=0.47, P < 0.01). Conclusions: Hydrogen-rich water and exercise prior to myocardial infarction can reduce myocardial interstitial fibrosis, promote cardiac function, and increase cardiac β3-AR and eNOS expression. The effect of hydrogen-rich water or exercise prior to myocardial infarction on cardiac β3-AR is better than combination intervention of hydrogen-rich water and exercise, and there is no difference of improving cardiac function among three interventions.
查看全文  查看/发表评论  下载PDF阅读器