胡琰茹,刘晓莉,乔德才.力竭运动大鼠苍白球内侧部神经递质浓度及相应受体表达变化研究[J].北京体育大学学报,2015,38(1):57-62+79.
力竭运动大鼠苍白球内侧部神经递质浓度及相应受体表达变化研究
Effect of Exhaustive Exercise on Neurotransmitters and Their Receptors in Rats’ Globus Pallidus
投稿时间:2014-07-06  
DOI:10.19582/j.cnki.11-3785/g8.2015.01.009
中文关键词:  关键词:力竭运动  大鼠  苍白球内侧部  神经递质  受体表达
英文关键词:Key words: exhaustive exercise  rat  GPi  neurotransmitter  receptor expression
基金项目:国家自然科学基金资助项目(项目编号:31171138)。通信作者:乔德才。
作者单位
胡琰茹 北京师范大学体育与运动学院北京 100875中南大学体育教研部湖南 长沙 410083 
刘晓莉 北京师范大学体育与运动学院北京 100875 
乔德才 北京师范大学体育与运动学院北京 100875 
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中文摘要:
      摘要:目的:通过观察一次性力竭运动过程中大鼠苍白球内侧部(GPi)神经递质谷氨酸(Glu)和γ-氨基丁酸(GABA)及相关受体表达水平的动态变化,探讨力竭运动过程中苍白球内侧部神经元电活动变化的可能机制。方法:选取雄性Wistar大鼠为实验对象,采用微透析-高效液相色谱联用技术,观察一次性力竭运动过程中大鼠苍白球内侧部神经元胞外Glu和GABA水平动态变化;采用免疫荧光双标技术,检测安静、力竭时和恢复90min时谷氨酸离子型2B受体(NR2B)和γ-氨基丁酸A型受体a1亚基(GABAAa -1)表达水平。结果:自主运动期大鼠苍白球内侧部神经元胞外Glu/GABA比值均显著下降(P<0.05),而力竭即刻其胞外Glu/GABA比值则显著升高(P<0.01)。与安静状态相比,苍白球内侧部在恢复90min时NR2B受体阳性细胞数量显著增加(P<0.05),力竭即刻和恢复90min时GABAAa-1受体阳性细胞数量显著减少(P<0.05,P<0.01)。结论:力竭运动过程中大鼠苍白球内侧部Glu、GABA浓度及NR2B、GABAAa-1受体蛋白表达的改变是导致该核团神经元兴奋性变化的原因之一。
英文摘要:
      Abstract: Objective: The purpose of this study was to reveal the possible mechanism of the neurons activity change in globus pallidus (GPi) during exhaustive exercise by observing the dynamic changes of glutamate (Glu) , γ-aminobutyric acid (GABA) and their receptors in internal segment in rats’ GPi during exhaustive exercise. Methods: Male Wistar rats were selected in this study. The expressions of the Glu and GABA in GPi during an exhaustive exercise were observed by microdialysis-high performance liquid chromatogram techniques. The expressions of NR2B receptor and GABA Aa -1 receptor were detected at rest, during exercise, 90 min after exercise, by double-lable immunofluorescence technique. Results: Compared with rest state, the Glu/GABA ratio of GPi significantly decreased during the auto-exercise period (P < 0.05), while it was significantly increased at exhaustion time point (P < 0.01). The NR2B positive cells in GPi were increased significantly at 90 min after exhaustive exercise compared with rest state (P < 0.05), but GABAAa-1 positive cells were decreased significantly both at exhaustion time point (P < 0.05) and 90 min after exhaustion (P < 0.01). Conclusion: The change of expressions of Glu, GABA, NR2B receptor and GABAAa-1 receptor in rats’ GPi during exhaustive exercise is a reason for neurons activity change.
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