刘霞,金其贯,罗强.运动和膳食控制对2型糖尿病大鼠脂联素-AMPK-GLUT4通路的影响[J].北京体育大学学报,2013,36(1):55-58.
运动和膳食控制对2型糖尿病大鼠脂联素-AMPK-GLUT4通路的影响
Effect of Exercise and Dietary Control on the Adiponectin-AMPK-GLUT4 Signaling Pathway in the Type-2 Diabetic Rats
投稿时间:2012-09-05  
DOI:
中文关键词:  关键词:2型糖尿病  有氧运动  膳食控制  脂联素  AMPK  GLUT4
英文关键词:Key words:type-2 diabetes  aerobic exercise  dietary control  adiponectin  AMPK  GLUT4
基金项目:基金项目:扬州大学科技培育基金。
作者单位
刘霞 扬州大学体育学院江苏 扬州 225009 
金其贯 扬州大学体育学院江苏 扬州 225009 
罗强 南京市六合区实验高级中学江苏 南京 211500 
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中文摘要:
      摘要:目的:研究运动和膳食控制对T2DM大鼠脂联素-AMPK-GLUT4的影响。方法:雄性SD大鼠62只,随机抽取8只大鼠作为正常对照组(C),喂以标准普通饲料。其余54只在喂饲高脂膳食的基础上,腹腔注射小剂量的STZ,建立T2DM动物模型,随机分成DM对照组(DM)、DM+运动锻炼组(DME)、DM +膳食控制组(DMD)、DM+运动锻炼+膳食控制组(DMED)4组。12周后,检测各组大鼠血清脂联素、肌糖原、骨骼肌GLUT4、AMPK含量。结果:1)与C组相比,DM组大鼠血清中脂联素显著下降(P<0.05),骨骼肌AMPK和GLUT4含量极显著性降低(P<0.01),肌糖原含量虽有下降,但无显著性差异(P>0.05)。2)运动可使DM大鼠肌糖原、血清脂联素和骨骼肌AMPK含量显著增加(P<0.05),虽使骨骼肌GLUT4含量增加,但没有显著性(P>0.05)。膳食控制可使DM大鼠骨骼肌肌糖原、GLUT4和AMPK含量增加,但均无显著性(P>0.05);但可使DM大鼠血清脂联素含量显著增加(P<0.05)。运动联合膳食控制对进一步增加DM大鼠血清脂联素和骨骼肌肌糖原无显著性的交互作用(P>0.05),但对增加骨骼肌AMPK和GLUT4含量有显著性交互作用(P<0.05)。结论:1)T2DM大鼠脂联素-AMPK-GLUT4信号通路受阻,在糖尿病发病中起着重要的作用。2)长期的有氧运动可显著增强T2DM大鼠脂联素-AMPK-GLUT4信号通路,单纯的膳食控制对改善T2DM大鼠脂联素-AMPK-GLUT4信号通路的作用不大,有氧运动联合膳食控制对增加T2DM大鼠骨骼肌AMPK和GLUT4有显著交互作用。
英文摘要:
      Abstract:Objective:To explore the effects of exercise and dietary control on adiponectin-AMPK-GLUT4 in T2DM rats. Methods: 8 rats were randomly selected from 62 male SD rats used as normal control group(C),fed with a standard diet. The remaining 54 were intraperitoneally injected small doses of STZ on the basis of feeding high fat diet to establish T2DM animal models. Then T2DM rats were randomly divided into 4 groups: DM group(DM),DM+ exercise group(DME): DM + diet control group(DMD),DM+ exercise + diet control group(DMED). After 12 weeks, the serum adiponectin concentration, the glycogen, GLUT4 and AMPK contents of skeletal muscle were measured. Results:1)Compared with group C,the serum adiponectin in DM group rats decreased significantly(P<0.05),the contents of AMPK and GLUT4 in skeletal muscle decreased more significantly(P< 0.01),the muscle glycogen content decreased too,but there was no significant difference(P>0.05).2)Aerobic exercise could increased serum adiponectin,muscle glycogen and AMPK content in DM rats significantly(P<0.05),although the skeletal muscle GLUT4 levels increased,but there was no significant difference(P>0.05). Dietary control could make glycogen,GLUT4 and AMPK contents in skeletal muscle of the DM rats increase,but there were no significant difference(P>0.05),but it could make serum adiponectin content in the DM rats increase significantly(P<0.05). 〖JP2〗Exercise and dietary control were no significant interaction on further increasing serum adiponectin and glycogen contents in DM rats’ muscle(P>0.05),but they had a significant interaction on AMPK and GLUT4 content in skeletal muscle(P<0.05). Conclusion:1)The adiponectin-AMPK-GLUT4 signaling pathway in T2DM rats was blocked,which played an important role in the onset of diabetes. 2)Long-term aerobic exercise could improve adiponectin-AMPK-GLUT4 signaling pathways in T2DM rats,simple dietary control could play a minor role on improving adiponectin-AMPK-GLUT4 signaling pathways in T2DM rats,aerobic exercise combined with diet control had a significant interaction on increasing AMPK and GLUT4 content of skeletal muscle in T2DM rats.〖JP〗
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