常娟,梁恒.运动和雌二醇对去卵巢大鼠GSK-3β蛋白表达的影响[J].北京体育大学学报,2012,35(12):62-66.
运动和雌二醇对去卵巢大鼠GSK-3β蛋白表达的影响
Effect of Exercise and Estradiol on GSK-3βExpression of Ovariectomized Rat
投稿时间:2012-04-16  
DOI:
中文关键词:  关键词:运动  雌激素  去卵巢  GSK-3β蛋白  血清E2  脂肪GSK  甘油三酯
英文关键词:Key words: exercise  estrogen  ovariectomized  GSK-3β  serum E2  axunge GSK  triglyceride
基金项目:基金项目:硕士学位论文。通信作者:梁恒。
作者单位
常娟 湖南师范大学体育学院湖南 长沙 410012 
梁恒 中山大学教育学院体育系广东 广州 510275 
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中文摘要:
      摘要:目的:建立一去卵巢大鼠模型,通过运动和体外补充雌激素,探讨运动和雌激素对去卵巢大鼠脂肪代谢中GSK-3β、P-GSK-3β的影响。方法:以雌性去卵巢SD大鼠为研究对象,进行为期15周的实验,随机分为假手术安静组(Sham)、假手术运动组(Sham+E)、去卵巢安静组(OVX)、去卵巢运动组(OVX+E)和去卵巢雌激素组(OVX+E2)。运动组大鼠18m/min,坡度5°,45min/d,每周运动5d;去卵巢激素组大鼠每周按体重25μg/kg注射E2,每周3次。实验结束后测定大鼠腹腔内脂肪重量,采用放射免疫方法测血清中雌激素E2水平,WesternBlot测定大鼠脂肪总蛋白含量和脂肪组织GSK-3β和P-GSK-3β蛋白表达。结果:1)经过15周实验后,去卵巢安静组大鼠体重显著高于假手术安静组(P<0.05),运动和补充雌激素之后体重明显下降(P<0.05);2)去卵巢安静组大鼠腹腔内脂肪重量最大,运动和雌激素补充干预后脂肪重量明显降低(P<0.05);3)大鼠去卵巢之后脂肪组织中甘油三酯含量显著升高(P<0.05),运动和补充雌激素后又显著下降(P<0.05);4)运动训练后,假手术运动组大鼠和去卵巢运动组大鼠脂肪组织P-GSK-3β/GSK-3β蛋白表达量均显著升高(P<0.05)。结论:1)运动训练和体外补充雌激素,在一定程度上可以缓解去卵巢大鼠腹腔内脂肪的积累;2)中等强度运动训练能够提高去卵巢大鼠体内雌激素水平;3)运动能够上调脂肪组织中P-GSK-3β/GSK-3β蛋白的表达;体外补充雌激素对P-GSK-3β/GSK-3β蛋白的表达却无明显影响。
英文摘要:
      Abstract: Objective: The present thesis designed an ovariectomized rat model, through the exercise and estrogen replacement therapy, to explore the effects of exercise and estrogen on GSK-3β, P-GSK-3β of lipid metabolism in ovariectomized rats. Methods: The ovariectomized female Sprague-Dawley rats, provided exercise and estrogen replacement for a period of 15 weeks, were randomly divided into five groups: Sham, Sham+E, OVX, OVX+E and OVX+E2. The exercise groups would performed 18m/min, gradient of 5 degree, 45min and five days a week; The OVX+E2 group was injected the estrogen according to the weight 25ug/kg weekly, three times a week. After the experiment, the researchers measured the intra-abdominal fat mass in rats, and measured serum levels of estrogen (E2) by radio-immunitied. The total protein content and adipose tissue GSK-3β and P-GSK-3β protein were determined through BCA and Western blot. Results:1) After 15-week experiment, the body weight in OVX was significantly higher than Sham (P<0.05), and after exercise and estrogen replacement therapy it decreased respectively(P<0.05). 2)after ovariectomized, the intra-abdominal fat OVX rats were the heaviest in all group, and through exercise and estrogen replacement, fat mass were decreased significantly(P<0.05). 3) the triglyceride content in OVX adipose tissue was significantly higher (P <0.05), after exercise and estrogen replacement therapy the triglyceride significantly decreased (P<0.05) 4) The exercise group P-GSK/GSK protein expression in adipose tissue were significantly higher than quiet group (P <0.05 ). Conclusions: 1) Exercise and estrogen replacement therapy in vitro can certain release the ovariectomized rat intra-abdominal fat accumulation. 2) Moderate-intensity exercise can improve the ovariectomized rats estrogen (E2) levels. 3) Exercise increase adipose tissue P-GSK-3β/GSK-3β protein expression, but estrogen couldn’t influence the P-GSK-3β/GSK-3β protein expression.
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