李珊珊,石丽君,李丽.有氧运动在改善胸主动脉平滑肌反应性中的KATP通道机制[J].北京体育大学学报,2012,35(12):45-48.
有氧运动在改善胸主动脉平滑肌反应性中的KATP通道机制
KATP Channel Mechanisms Underlying the Aerobic Exercise-induced Improvement of the Reactivity of the Vascular Smooth Muscle in Thoracic Aorta
投稿时间:2012-06-04  
DOI:
中文关键词:  关键词:有氧运动  胸主动脉  平滑肌  KATP通道  血管收缩  血管舒张
英文关键词:Key words: aerobic exercise  thoracic aorta  smooth muscle  KATP channels
基金项目:基金项目:教育部新世纪优秀人才培养资助(NCET-11-0850);国家自然科学基金(31071033);国家科技支撑计划课题(2012BAK21B03)资助。通信作者:石丽君。
作者单位
李珊珊 北京体育大学北京 100084 
石丽君 北京体育大学北京 100084 
李丽 北京体育大学北京 100084 
摘要点击次数: 2962
全文下载次数: 275
中文摘要:
      摘要:目的:探讨有氧运动对大鼠胸主动脉平滑肌细胞KATP通道的影响。方法:Wistar大鼠,雄性,2月龄,共20只,随机分为2组,安静对照组和有氧运动组,运动组进行8周递增速度跑台运动。8周后,在体实验各组选取5只,进行股动静脉插管手术,术后恢复1d后,测定注射KATP通道阻断剂glibenclamide后的血压和心率;剩余10只大鼠进行离体实验,大鼠开胸腔取胸主动脉,去内皮,制备成血管环,分别给予KATP通道阻断剂glibenclamide和KATP通道开放剂pinacidil等药物刺激,观察两种药物所引起的血管反应性变化。结果:1)运动组静脉注射KATP通道阻断剂glibenclamide(20 mg/kg)所引起的血压升高幅度显著增大,心率显著下降。2)glibenclamide(3×10-5M)可诱发大鼠血管张力增加,运动后其升高幅度显著高于安静组。3)有氧运动组pinacidil诱导的血管舒张反应显著增大。结论:有氧运动在改善胸主动脉血管平滑肌反应性的作用中,KATP通道活性的增强是重要机制之一。
英文摘要:
      Abstract: Objective: To investigate the effect of aerobic exercise training on the KATP channel in the thoracic aortic smooth muscle cells. Methods: Male Wistar rats (2M), were randomly divided into two groups, sedentary control group and aerobic exercise training group. Aerobic exercise training on the treadmill was applied on aerobic exercise training group for 8 weeks. After 8 weeks, some animals (n=5 in each group) underwent femoral arterial and venous cannulation. Following 1 day recovery, the blood pressure and the heart rate were mensurated after injection of glibenclamide which is the specific blocker of KATP channel. In vitro study, the thoracic aorta was collected, the endothelium was removed, and the vascular rings were prepared. Rats were respectively infused with specific KATP channel blocker glibenclamide and specific KATP channel opener pinacidil. Then, the changes of vessel response were measured. Results: 1) The pressor responses induced by i.v. injection of KATP channel blocker glibenclamide (20mg/kg) was significantly enhanced after exercise training; the heart rate of aerobic exercise training group was decreased significantly after injection of glibenclamide. 2) Glibenclamide(3×10-5M) could induce an increase of vessel contractility, and the amplitude of increase was significantly enhanced after exercise training. 3) In aerobic exercise training group, the vessel dilation induced by pinacidil was more significant than that of sedentary control group. Conclusion: The enhancement of KATP channel may be one of the important mechanisms underlying the improvement of the vascular smooth muscle reactivity in thoracic aorta.
查看全文  查看/发表评论  下载PDF阅读器