张琳,郝选明.胸腺细胞转录因子TCF-1、Nur77以及mRNA对长期递增负荷运动的应答性特征[J].北京体育大学学报,2012,35(3):56-59.
胸腺细胞转录因子TCF-1、Nur77以及mRNA对长期递增负荷运动的应答性特征
Responsive Characteristics of the Thymocyte Transcription Factor TCF-1, Nur77 and their mRNA on Long Term Load Incremental Exercises
投稿时间:2011-06-14  
DOI:
中文关键词:  T细胞特异性转录因子-1  Nur77  递增负荷运动  胸腺
英文关键词:T cell specific transcription factor 1(TCF 1)  Nur77  incre mental exercise  thymus
基金项目:中国博士后科学基金(NO:20110490909);广东省 自然科学基金(NO:S2011040003164);广东省自然科学基金(NO:9151063101000059)
作者单位
张琳 华南师范大学广东 广州 510631 
郝选明 华南师范大学广东 广州 510631 
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中文摘要:
      目的:通过研究对胸腺细胞发育具有正性调节的作用的转录因子TCF-1和负性调节作 用的Nur77在6周递增负荷运动的应答性特征,探讨长期运动影响胸腺细胞发育的调节机制 。方法: 128只SD大鼠随机分为运动组和对照组,运动组进行递增负荷跑台训练6周,每周6 次,每天30 min。分别于第0、2、4、6周末利用IHC、WB及FQ-RT-PCR技术 测定安静状态、运动后即刻和运动后3 h胸腺TCF-1、Nur77及其mRNA表达水平。结果:TCF -1和Nur77呈现几乎相反的应答性变化:1) TCF-1:对负荷初次应答时运动前后没有明显变 化;第2周末和第四周末运动后明显降低,恢复3 h升高,呈“V”型应答曲线;第6周末, 则在运动后持续下降。其mRNA应答趋势与其蛋白表达相一致。2) Nur77:第0、2、4周末呈 现倒“V”型变化,第6周则持续上升。其mRNA应答趋势与其蛋白表达相一致。结论:随着递 增负荷运动的进程,TCF-1及其mRNA总体水平越来越低,且越来越难以恢复,表明对胸腺细 胞发育的正性调节作用越来越弱;Nur77及其mRNA总体水平越来越高,恢复也越来越难,表 明对胸腺细胞的负性调节作用越来越强。这应该是胸腺细胞受长期递增负荷运动影响,胸腺 细胞发育受阻且功能逐步下降的重要调节机理之一。
英文摘要:
      Objective:This study tried to observe responsive characteristics of the positiv e regulatory factor, TCF 1, and negative factor, Nur77, to thymus lymphocyte de velopment during six weeks incremental exercise so as to shed some light on reg ulative mechanism of thymocyte development with long term exercise. Method: 128 rats, were randomly divided into control and exercise group. Exercise protocol: the exercise group conducted load incremental step running exercise for six wee ks, 30 minute a day and six times a week.Samples were taken before ex ercise and in the end of WK2, 4 and 6, at rest, just after exercise and 3 hours after exercise, respectively. TCF 1, Nur77 and their mRNA in thymus were measur ed by IHC, WB and FQ RT PCR. Result: TCF 1 and Nur77 presented almost opposit ely response during six week incremental exercise: 1) TCF 1: no distinguished changes occurred in pre and post exercise, and “V” responsive patter n were observed in week 2 and 4. And its mRNA showed similar changing pattern. 2 ) Nur77: response showed an inversed “V” pattern in the end of WK0,WK2 and WK4 . Conclusion: With incremental exercise, level of TCF 1 and mRNA were gradually decreased, and showed more difficult recovery while Nur77 and mRNA showed o pposite change. This indicated down regulation to thymus lymphocyte development was stronger and stronger with exercise, on the contrast, up regulation weaker and weaker. As result of interaction of two factors, lymphocyte’s development was seriously inhibited. This should be one of the important mechanisms of exerc ise induced immunosuppression.
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